Composite

Part:BBa_K4759210:Design

Designed by: jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS5-hemA-RBS2-hemL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2406
    Illegal NheI site found at 2116
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2406
    Illegal BglII site found at 331
    Illegal BglII site found at 609
    Illegal BamHI site found at 1343
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1349
    Illegal EcoRI site found at 2406
    Illegal AgeI site found at 1766
    Illegal AgeI site found at 2099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 263


Design Notes

We constructed the recombinant plasmid pCDFDuet-hemA-hemL to express hemA and hemL in engineered bacteria, hemA and hemL are use RBS2 to link to under the promoter to express.

Source

E.coli genome


References

[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.